Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Usage Physical State: Freeze-dried solidStorage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ConjugateHorseradish Peroxidase
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Images & ReferencesThis product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
SERO——自1963年起独立对照血清生产专家
SERO是挪威一家专业的生物技术公司。专业研发质量控制材料，数十年来通过为全球医疗实验室行业提供高水平的质控产品，而对诊断行业做出杰出贡献。
早在1950年，医学实验室质控领域的先驱，有丰富的医学实验室质控经验的伦兹艾尔杰恩教授，凭借渊博的质控知识开始开发对照血清，并在1963年建立了SERO。近年来，随着认证程序的要求，许多国家对国家授权的推荐，越来越多的实验室意识到使用完全不同于他们仪器和试剂来源的第三方对照的重要性，这使高品质独立对照产品受到越来越多的重视，SERO品牌的对照产品也受到国际上越来越多的欢迎。
＞SERO产品
SERO生产以下系列专利产品Seronorm™, Pathonorm™ and Autonorm™。除此之外，也为客户提供个性化定制产品。
＞Seronorm™系列
主要产品包括免疫对照，结合激素，心脏和肿瘤标志物，Seronorm™所有对照血清来源为人，不含防腐剂和稳定剂，产品文档全面，为市场上最常用的仪器提供超过50种分析物的分配值，可提供四个不同临床级别，且每一级别都可单独提供。Seronorm™ Immunoassay Liq low – 含有与临床相当的低水平的醛固酮，雄烯二酮，皮质醇，铁蛋白，IgE，17-OH-孕酮，PSA总量，睾酮，游离T4，TSH，25-OH-维生素D和维生素B12
＞Pathonorm™ and Autonorm™
Pathonorm™临床化学级对照，不含防腐剂和稳定剂的动物血清，适合大部分临床化学分析，为市面上常用仪器提供超过38种分析物的分配值，有两种临床级别，且都可以单独提供，产品以冻干粉形式提供，货架期长。
Autonorm™
满足绝大部分临床化学实验室正常水平的分析级的高品质对照，不含防腐剂和稳定剂的动物血清，不含Pharmaca。文档齐全，为市场上最常用的仪器提供超过30种分析物的分配值，冻干粉，货架期长。
客户定制产品：
SERO也针对有质量意识的诊断公司，组织提供定制服务，SERO可定制的高品质对照产品如下：
＞常规血清化学（人或动物基质）
＞常规尿液化学
＞脂质
＞激素
＞肿瘤标记
＞心脏标志物
＞蛋白质
＞治疗药物
＞专业和护理点控制（CRP，HbA1C，血红蛋白，葡萄糖）
＞微量元素和重金属
目前世界上约有60多个国家可以提供高品质的对照，SERO是这个领域的先驱也是世界领先的对照血清生产者
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